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CAPN1 Antibody#31038

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Product Detail

Product NameCAPN1 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

ApplicationsELISA WB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of total CAPN1 protein.

Immunogen TypeRecombinant Protein

Immunogen DescFusion protein corresponding to a region derived from 543-713 amino acids of Human Calpain-1 catalytic subunit

Target NameCAPN1

ConjugateUnconjugated

Other NamesCalpain-1 catalytic subunit, CANP, muCL, CANP1, CANPL1, muCANP

Accession NoSwiss-Prot:P07384
Gene ID:823;

Uniprot P07384

Gene ID 823;

FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

StorageStore at -20˚C/1 year

Application Details

Predicted MW: 82kd
ELISA: 1:1000-1:5000
Western blotting: 1:200-1:1000

Gel: 10%SDS-PAGE
Lysate: 30 µg 293T cell lysate
Primary antibody: 1/450 dilution
Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution
Exposure time: 1 minute

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.

If you have published an article using product 31038, please notify us so that we can cite your literature.

et al,TAT?fused IP3R?derived peptide enhances cisplatin sensitivity of ovarian cancer cells by increasing ER Ca2+ release.In Int J Mol Med. On 2018 Feb by Xie Q, Xu Y et al..PMID: 29207009 , (2018),
PMID: 29207009
et al,Bcl-2 overexpression reduces cisplatin cytotoxicity by decreasing ER-mitochondrial Ca2+ signaling in SKOV3 cells.In Oncol Rep.On 2018 Mar by Xu L, Xie Q et al..PMID:29286126 , (2018),
PMID: 29286126

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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