Product Detail
Product NameNAMPT Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were purified by affinity purification using immunogen.
ApplicationsWB,IHC,IF
Species ReactivityHuman,Mouse,Rat
SpecificityThe antibody detects endogenous level of total NAMPT protein.
Immunogen TypeRecombinant Protein
Immunogen DescRecombinant protein of human NAMPT.
Target NameNAMPT
ConjugateUnconjugated
Other NamesNAMPT; 1110035O14Rik; DKFZp666B131; MGC117256; PBEF
Accession NoSwiss-Prot:P43490
NCBI Gene ID:10135
Uniprot
P43490
Gene ID
10135;
Sdspage MW52KD
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Immunohistochemistry of paraffin-embedded rat ovary using PBEF / NAMPT at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using PBEF / NAMPT at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using PBEF / NAMPT at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using PBEF / NAMPT at 1:1000 dilution.
Immunofluorescence analysis of HeLa cells using PBEF / NAMPT .
Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.
If you have published an article using product 32047, please notify us so that we can cite your literature.
et al,Naoxinqing tablet protects against cerebral ischemic/reperfusion injury by regulating ampkα/NAMPT/SIRT1/PGC-1α pathwayInJ EthnopharmacolOn2024 Mar 25byXiao Sun?1,?Yunfeng Pan et al..PMID:?38159826
, (2023),
PMID:
38159826
et al,Naoxinqing tablet protects against cerebral ischemic/reperfusion injury by regulating ampkα/NAMPT/SIRT1/PGC-1α pathway. In J Ethnopharmacol on 2024 Mar 25 by Xiao Sun, Yunfeng Pan,et al..PMID:38159826
, (2024),
PMID:
38159826
Yes
