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IRS-1 (Ab-312) Antibody#33191

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Product Detail

Product NameIRS-1 (Ab-312) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC IF

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous levels of total IRS-1 protein.

Immunogen TypePeptide

Immunogen DescSynthesized non-phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 312 (A-T-S(p)-P-A).

Target NameIRS-1

ConjugateUnconjugated

Other NamesInsulin receptor substrate-1; IRS-1; IRS1;

Accession NoSwiss-Prot: P35568
NCBI Gene ID: 3667

Uniprot P35568

Gene ID 3667;

Sdspage MW180kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IRS-1 (Ab-312) antibody #33191.
Immunofluorescence analysis of HeLa cells, using IRS-1 (Ab-312) antibody #33191.
Western blot analysis of extracts from NIH-3T3 cells, using IRS-1 (Ab-312) antibody #33191.
Western blot analysis of extracts from JK cells (Lane 2), using IRS-1 (Ab-312) antiobdy #33191. The lane on the left is treated with synthesized peptide.
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit By similarity.

Araki E., Diabetes 42:1041-1054(1993).
Nishiyama M., Biochem. Biophys. Res. Commun. 183:280-285(1992).
The MGC Project Team; Genome Res. 14:2121-2127(2004)

If you have published an article using product 33191, please notify us so that we can cite your literature.

et al,Liraglutide Ameliorates HyperhomocysteInemia-Induced Alzheimer-Like Pathology and Memory Deficits in Rats via Multi-molecular TargetIng. In Neurosci Bull on 2019 Jan 10 by Zhang Y, Xie JZ, et al..PMID: 30632006 , (2019),
PMID: 30632006

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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