Product Detail
Product NameiNOS (Ab-151) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous levels of total iNOS protein.
Immunogen TypePeptide
Immunogen DescSynthesized non-phosphopeptide derived from human iNOS around the phosphorylation site of tyrosine 151 (Q-Y-Y(p)-G-S).
Target NameiNOS
ConjugateUnconjugated
Other NamesEC 1.14.13.39; HEP-NOS; hepatocyte NOS; inducible NOS; nitric oxide synthase
Accession NoSwiss-Prot: P35228
NCBI Gene ID: 4843
Uniprot
P35228
Gene ID
4843;
Sdspage MW131kd
Concentration1.0mg/ml
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:500~1:3000
Western blot analysis of extracts from 3T3 cells, using iNOS (Ab-151) antibody #33261.
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
Geller D.A., Proc. Natl. Acad. Sci. U.S.A. 90:3491-3495(1993).
Sherman P.A., Biochemistry 32:11600-11605(1993).
Charles I.G., Proc. Natl. Acad. Sci. U.S.A. 90:11419-11423(1993)
If you have published an article using product 33261, please notify us so that we can cite your literature.
et al,Xanthohumol exerts protective effects in liver alterations associated with agIng. In Eur J Nutr on 2019 Mar by Fern ndez-Garc a C, Rancan L, et al.. PMID: 29536163
, (2019),
PMID:
29536163
et al,HMGN2 regulates non-tuberculous mycobacteria survival via modulation of M1 macrophage polarization. In J Cell Mol Med on 2019 Dec by Wang X, Chen S, et al..PMID:31596045
, (2019),
PMID:
31596045
et al,Clonorchis sinensis ferritin heavy chain triggers free radicals and mediates inflammation signaling in human hepatic stellate cells.In Parasitol Res on 2015 Feb by Qiang Mao, Zhizhi Xie et al..PMID: 25413629
, (2015),
PMID:
25413629
et al,The Effects of Xiangqing Anodyne Spray on treating acute soft-Tissue injury mainly depend on suppressing activations of AKT and p38 pathways.In Evid Based Complement Alternat Med on 2016 by Shudong Wang , Tao Li et al..PMID:27190541
, (2016),
PMID:
27190541
Yes
