Product Detail
Product NameCox2 Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB IHC IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous levels of total Cox2 protein.
Immunogen TypePeptide
Immunogen DescSynthesized peptide derived from human Cox2.
Target NameCox2
ConjugateUnconjugated
Other NamesCOX2; GRIPGHS; PGHS2; CYCLOOXYGENASE 2; GLUCOCORTICOID-REGULATED INFLAMMATORY PROSTAGLANDIN G/H SYNTHASE
Accession NoSwiss-Prot: P35354
NCBI Gene ID: 5743
Uniprot
P35354
Gene ID
5743;
Sdspage MW80kd
Concentration1.0mg/ml
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cox2 antibody #33345.
Immunofluorescence analysis of A549 cells, using Cox2 antibody #33345.
Western blot analysis of extracts from A549 cells, using Cox2 antibody #33345.
Western blot analysis of extracts from HeLa cells (Lane 2), and A549 cells (Lane 3), using Cox2 antiobdy #33345. The lane on the left is treated with systhesized peptide.
Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.
Nathalie Bonnefoy, Mol. Cell. Biol., Apr 2001; 21: 2359 - 2372.
Keith P. Choe, J. Exp. Biol., May 2006; 209: 1696 - 1708
D. Shelley Lupold, PNAS, Sep 1999; 96: 11670.
Axel J. Baumgarten, AACR Meeting Abstracts, Mar 2004; 2004: 1078.
If you have published an article using product 33345, please notify us so that we can cite your literature.
et al,Danzhi Jiangtang Capsule ameliorates kidney injury via inhibition of the JAK-STAT signaling pathway and increased antioxidant capacity in STZ-induced diabetic nephropathy rats.In Biosci Trends.On 2019 Jan 22 by Sun M, Bu W et al..PMID: 30606979
, (2018),
PMID:
30606979
et al,Responses of inflammation signaling pathway by saucerneol D from elicitor-treated Saururus chinensis on pro-inflammatory responses in LPS-stimulated Raw 264.7 cell.
, (2021),
PMID:
et al,Anti-inflammatory Effect of Wild Indigo (Baptisia tinctoria) Root on Raw 264.7 Cells with Stimulated Lipopolysaccharide
, (2022),
PMID:
et al,Combined Administration of Curcumin and Chondroitin Sulfate Alleviates Cartilage Injury and Inflammation via NF-κB Pathway in Knee Osteoarthritis Rats. In Front Pharmacol on 2022 May 19 by Ting Guan, Liu-Gang Ding, et al..PMID: 35662715
, (2022),
PMID:
35662715
et al,ROS-Sp1 axis is involved in thermochemotherapy-enhanced sensitivity of pancreatic cancer cells to gemcitabineInCell Biol IntOn2023 NovbyPan Xi?1?2,?Xiaojuan Ma et al..PMID: 37545170
, (2023),
PMID:
37545170
et al,Iridium nanozyme-mediated photoacoustic imaging-guided NIR-II photothermal therapy and tumor microenvironment regulation for targeted eradication of cancer stem cellsInActa BiomaterOn2023 DecbyYuying Yao , Zhuangzhuang Zhao et al..PMID:37852456
, (2023),
PMID:
37852456
et al,Hepatoprotective effects of Xiaoyao San formula on hepatic steatosis and inflammation via regulating the sex hormones metabolism. In World J Hepatol on 2024 Jul 27 by Xiao-Li Mei , Shu-Yi Wu ,et al..PMID:39086531
, (2024),
PMID:
39086531
More
et al,Gut microbiota analysis and LC-MS-based metabolomics to investigate AMPK/NF-κB regulated by Clostridium butyricum in the treatment of acute pancreatitis. In J Transl Med on 2024 Nov 27 by Biyan Wen, Yaoxing Huang, et al..PMID:39604956
, (2024),
PMID: 39604956
Yes
