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GAD67 Antibody#33379

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Product Detail

Product NameGAD67 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total GAD67 protein.

Immunogen TypePeptide

Immunogen DescSynthesized peptide derived from human GAD67.

Target NameGAD67

ConjugateUnconjugated

Other Names4.1.1.15; GLUTAMATE DECARBOXYLASE; BRAIN; 67-KD; glutamate decarboxylase 1 (brain

Accession NoSwiss-Prot: Q99259
NCBI Gene ID: 2571

Uniprot Q99259

Gene ID 2571;

Sdspage MW67kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human brain tissue using GAD67 antibody #33379.
Western blot analysis of extracts from LOVO cells, using GAD67 antibody #33379.
Western blot analysis of extracts from A549 cells (Lane 2) and HepG2 cells (Lane 3), using GAD67 antiobdy #33379. The lane on the left is treated with systhesized peptide.
Catalyzes the production of GABA.

Jamil Kanaani, J. Biol. Chem., Dec 1999; 274: 37200.
Susan M. Murphy, J. Histochem. Cytochem., Nov 1998; 46: 1261.
M. Gierdalski, Cereb Cortex, Sep 2001; 11: 806 - 815.
Kyung Soo Kim, Diabetes, Sep 2002; 51: 2764.

If you have published an article using product 33379, please notify us so that we can cite your literature.

et al,Increased GluK1 Subunit Receptors in Corticostriatal Projection from the Anterior Cingulate Cortex Contributed to Seizure‐Like Activities. In Adv Sci (Weinh) on 2024 Oct by Xu-Hui Li, Wantong Shi, et al..PMID:39225597 , (2024),
PMID: 39225597

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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