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HSP10 Antibody#33405

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Product Detail

Product NameHSP10 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total HSP10 protein.

Immunogen TypePeptide

Immunogen DescSynthesized peptide derived from human HSP10.

Target NameHSP10

ConjugateUnconjugated

Other NamesCHAPERONIN 10 HOMOLOG; GroES HOMOLOG; HEAT-SHOCK 10-KD PROTEIN; cpn10 HOMOLOG; heat shock 10kDa protein 1 (chaperonin 10)

Accession NoSwiss-Prot: P61604
NCBI Gene ID: 3336

Uniprot P61604

Gene ID 3336;

Sdspage MW10kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP10 antibody #33405.
Immunofluorescence analysis of NIH/3T3 cells, using HSP10 antibody #33405.
Western blot analysis of extracts from NIH/3T3 cells, using HSP10 antibody #33405.
Western blot analysis of extracts from COS7 cells (Lane 2), using HSP10 antiobdy #33405. The lane on the left is treated with systhesized peptide.
Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. HAMAP-Rule MF_00580

Yue-xin Shan, J. Biol. Chem., Nov 2003; 278: 45492 - 45498.
David LaVerda, Infect. Immun., Jan 2000; 68: 303 - 309.
K?re L. Nielsen, J. Bacteriol., Sep 1999; 181: 5871 - 5875.
Kurt M. Lin, Circulation, Apr 2001; 103: 1787 - 1792.

If you have published an article using product 33405, please notify us so that we can cite your literature.

et al,NAD+ repletion Inhibits the endothelial-to-mesenchymal transition Induced by TGF-β In endothelial cells through improvIng mitochondrial unfolded proteIn response. In Int J Biochem Cell Bio on 2019 Dec by Zhang M, Weng H, et al..PMID:31626975 , (2019),
PMID: 31626975

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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