Product Detail
Product NameHistone H2AX Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB IF
Species ReactivityHu
SpecificityThe antibody detects endogenous levels of total Histone H2AX protein.
Immunogen TypePeptide
Immunogen DescSynthesized peptide derived from internal of human Histone H2AX.
Target NameHistone H2AX
ConjugateUnconjugated
Other NamesH2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X
Accession NoSwiss-Prot: P16104
NCBI Gene ID: 3014
Uniprot
P16104
Gene ID
3014;
Sdspage MW15kd
Concentration1.0mg/ml
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Western blot analysis of extracts from HT-29 cells, using Histone H2AX antibody #33686.
Immunofluorescence analysis of COS7 cells, using Histone H2AX antibody #33686.
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Rogakou E.P., J. Biol. Chem. 273:5858-5868(1998).
Rogakou E.P., J. Biol. Chem. 275:9390-9395(2000).
If you have published an article using product 33686, please notify us so that we can cite your literature.
et al,ATR activated by EB virus facilitates chemotherapy resistance to cisplatin or 5-fluorouracil in human nasopharyngeal carcInoma. In Cancer Manag Res on 2019 Jan 9 by Zhang B, Cui B, et al..PMID:30666155
, (2019),
PMID:
30666155
et al,Downregulation and translocation of nuclear ING4 is correlated with tumorigenesis and progression of head and neck squamous cell carcinoma.In Oral Oncol on 2011 Mar by Li XH, Kikuchi K,et al..PMID:21310648
, (2011),
PMID:
21310648
Yes
