Product Detail
Product NameGrp78 Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were purified by antigen-affinity chromatography.
ApplicationsWB
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous levels of total Grp78 protein.
Immunogen TypeRecombinant Protein
Immunogen DescRecombinant fragment corresponding to a region within amino acids 478 and 654 of Grp78.
Target NameGrp78
ConjugateUnconjugated
Other NamesBIP antibody; FLJ26106 antibody; GRP78 antibody; MIF2 antibody; HSPA5 antibody; immunoglobulin heavy chain-binding protein antibody; 78 kDa glucose-regulated protein antibody; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody; "heat shoc
Accession NoSwiss-Prot#:P11021;
NCBI Gene#:3309
Uniprot
P11021
Gene ID
3309;
Sdspage MW72kd
Concentration1mg/ml
FormulationRabbit IgG in 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C
Application Details
Western blotting: 1:500-1:3000
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
#35394 diluted at 1:2000
Grp78 antibody detects Grp78 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
#102567 dilution: 1:1000
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]
If you have published an article using product 35394, please notify us so that we can cite your literature.
et al,Ginsenosides reduce body weight and ameliorate hepatic steatosis in high fat diet?induced obese mice via endoplasmic reticulum stress and p?STAT3/STAT3 signaling.In Mol Med Rep on 2020 Mar; by Yao Y et al..PMID:32016448
, (2020),
PMID:
32016448
Yes
