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ACTB Antibody#35532

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Product Detail

Product NameACTB Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntigen affinity purification.

ApplicationsWB

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous levels of total ACTB protein.

Immunogen TypePeptide

Immunogen DescSynthetic peptide corresponding to residues near the N terminal of human Actin, beta

Target NameACTB

ConjugateUnconjugated

Other NamesBRWS1; PS1TP5BP1

Accession NoSwiss-Prot#: P60709
NCBI Gene ID: 60
Gene Accssion: NP_001092

Uniprot P60709

Gene ID 60;

Sdspage MW42kd

Concentration1.6mg/ml

FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:1000-1:5000
Gel: 10%SDS-PAGE
Lysates (from left to right): Hela, Jurkat and NIH/3T3 cell, human fetal liver and mouse brain tissue, A431 and 293T cell
Amount of lysate: 40ug per lane
Primary antibody: 1/600 dilution
Secondary antibody dilution: 1/8000
Exposure time: 7 seconds
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs.

If you have published an article using product 35532, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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