Product Detail
Product Name6-His Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntigen affinity purification.
ApplicationsWB
SpecificityThe antibody detects transfected proteins contanining 6-His tag.
Immunogen TypePeptide
Immunogen DescSynthetic peptide: 18XHis conjugated to KLH
Target Name6-His
ConjugateUnconjugated
Other Names6 His epitope tag
Sdspage MW46kd
Concentration0.5mg/ml
FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:500-1:2000
Gel: 8%SDS-PAGE
Lysate: 2ug Recombinant 6-HIS Tagged protein
Primary antibody: 1/500 dilution
Secondary antibody dilution: 1/8000
Exposure time: 5 seconds
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.
If you have published an article using product 35533, please notify us so that we can cite your literature.
et al,VPS36-Dependent Multivesicular Bodies Are Critical for Plasmamembrane Protein Turnover and Vacuolar Biogenesis.In Plant Physiol on 2017 Jan by Huei-Jing Wang , Ya-Wen Hsu,et al..PMID: 27879389
, (2017),
PMID:
27879389
et al,Dual mechanisms regulating glutamate decarboxylases and accumulation of gamma-aminobutyric acid in tea (Camellia sinensis) leaves exposed to multiple stresses.In Sci Rep on 2016 Mar 29 by Xin Mei , Yiyong Chen et al..PMID:27021285
, (2016),
PMID:
27021285
et al,Dual mechanisms regulating glutamate decarboxylases and accumulation of gamma-aminobutyric acid in tea ( Camellia sinensis ) leaves exposed to multiple stresses
, (2016),
PMID:
Yes
