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GFP Antibody#35538

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Product Detail

Product NameGFP Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntigen affinity purification.

ApplicationsWB

SpecificityThe antibody detects transfected proteins contanining GFP tag.

Immunogen TypeRecombinant Protein

Immunogen DescFull length fusion protein

Target NameGFP

ConjugateUnconjugated

Other NamesGFP-like chromoprotein

Accession NoGene Accssion: P42212

Sdspage MW27kd

Concentration1.2mg/ml

FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

StorageStore at -20˚C

Application Details

Western blotting: 1:1000-1:10000

Gel: 8%SDS-PAGE
Lysate: 20ug GFP transfected 293T cell
Primary antibody: 1/800 dilution
Secondary antibody dilution: 1/8000
Exposure time: 30 seconds
Gel: 8%SDS-PAGE
Lysates: 20ug GFP transfected 293T cell per lane
Primary antibody: at various dilution
Secondary antibody dilution: 1/8000
Exposure time: 30 seconds

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.

If you have published an article using product 35538, please notify us so that we can cite your literature.

et al,Loss of the TRPM4 channel in humans causes immune dysregulation with defective monocyte migration. In J Allergy Clin Immunol on 2024 Sep by Fang Yu, Satanay Hubrack,et al..PMID:38750824 , (2024),
PMID: 38750824

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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