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APAF1 Antibody#36718

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Product Detail

Product NameAPAF1 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntigen affinity purification.

ApplicationsWB

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous levels of total APAF1 protein.

Immunogen TypePeptide

Immunogen DescSynthetic peptide corresponding to residues near the C terminal of human Apoptotic protease-activating factor 1

Target NameAPAF1

ConjugateUnconjugated

Other NamesCED4

Accession NoSwiss-Prot#: O14727
NCBI Gene ID: 317
Gene Accssion: NP_863659

Uniprot O14727

Gene ID 317;

Sdspage MW38kd

Concentration0.5mg/ml

FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

StorageStore at -20˚C

Application Details

Western blotting: 1:200-1:1000

Gel: 8%SDS-PAGE
Lysate: 30ug Hela cell
Primary antibody: 1/150 dilution
Secondary antibody dilution: 1/8000
Exposure time: 1 minute
Western blotting analysis using APAF1 Antibody #36718.

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

If you have published an article using product 36718, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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