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ERP29 Antibody#37555

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Product Detail

Product NameERP29 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntigen affinity purification.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total ERP29 protein.

Immunogen TypePeptide

Immunogen DescSynthetic peptide corresponding to a region derived from internal residues of human endoplasmic reticulum protein 29

Target NameERP29

ConjugateUnconjugated

Other NamesERp28; ERp31; PDIA9; PDI-DB; C12orf8

Accession NoSwiss-Prot#: P30040
NCBI Gene ID: 10961
Gene Accssion: NP_006808

Uniprot P30040

Gene ID 10961;

Sdspage MW29kd

Concentration2.6mg/ml

FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200
Gel: 10%SDS-PAGE
Lysates (from left to right): Hela cell and human fetal liver tissue, A549 and 293T cell
Amount of lysate: 40ug per lane
Primary antibody: 1/200 dilution
Secondary antibody dilution: 1/8000
Exposure time: 40 seconds
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using #37555 at dilution 1/40.
This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms

If you have published an article using product 37555, please notify us so that we can cite your literature.

et al,miR-205-5p downregulation decreases gemcitabIne sensitivity of breast cancer cells via ERp29 upregulation. In Exp Ther Med on 2019 Nov by Ma C, Shi X, et al..PMID:31602229 , (2019),
PMID: 31602229

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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