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α-tubulin Mouse Monoclonal Antibody#37981

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Product Detail

Product Nameα-tubulin Mouse Monoclonal Antibody

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB,IHC,IF

Species ReactivityHu Rt Ms

SpecificityThe α-tubulin antibody can detects endogenousα-tubulin protein.

Target Nameα-tubulin

ConjugateUnconjugated

Other NamesTUBA1A;TUBA3;Tubulin alpha-1A chain;Alpha-tubulin 3;Tubulin B-alpha-1;Tubulin alpha-3 chain

Accession NoSwiss-Prot:Q71U36
Gene ID:7846

Uniprot Q71U36

Gene ID 7846;

Sdspage MW52kd

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details

WB dilution: 1:5000~1:10000
IHC dilution:1:200 IF dilution: 1:100~1:200
IP dilution: 1:200

Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue, using #37981 diluted at 1:5,000.
IF analysis of?Hela?with #37981(Left) and DAPI (Right) diluted at 1:100.
1¡¢Input: Mouse Brain Tissue Lysate
2¡¢IP product: IP dilute 1:200
Western blot analysis: primary antibody : #37981 1:5,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000
Immunofluorescence analysis of Human-colon-cancer tissue. 1,-tubulin Monoclonal Antibody(8F11)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-liver tissue. 1,-tubulin Monoclonal Antibody(8F11)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,-tubulin Monoclonal Antibody(8F11) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,-tubulin Monoclonal Antibody(8F11) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,-tubulin Monoclonal Antibody(8F11) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 KD. Microtubules are assembled from dimers of α- and β-tubulin

If you have published an article using product 37981, please notify us so that we can cite your literature.

et al,Alpha-lipoic acid alleviates NAFLD and triglyceride accumulation in liver via modulating hepatic NLRP3 inflammasome activation pathway in type 2 diabetic rats. In Food Sci Nutr on 2021 Mar 13 by Chih-Yuan Ko, Yangming Martin Lo, et al..PMID: 34026086 , (2021),
PMID: 34026086
et al,Promotion effect of TGF-β-Zfp423-ApoD pathway on lip sensory recovery after nerve sacrifice caused by nerve collateral compensationInInt J Oral SciOn2023 Jun 8byPingchuan Ma?#?1,?Gaowei Zhang? et al..PMID: 37286538 , (2023),
PMID: 37286538
et al,Novel oxicam nonsteroidal compound XK01 attenuates inflammation by suppressing the NF-κB and MAPK pathway in RAW264.7 macrophages. In Heliyon on 2024 Jan 9 by Jixiang Wang, Jiawang Tan,et al..PMID:38312593 , (2024),
PMID: 38312593

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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