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Location： Home > Loading Controls > GAPDH Rabbit Polyclonal Antibody

GAPDH Rabbit Polyclonal Antibody#37985

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Product Detail

Product NameGAPDH Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAffinity purification using immunogen.

ApplicationsWB,IHC

Species ReactivityHu Rt Ms Mk Chk Hm Sh Xenopus

SpecificityAntibody detects endogenous GAPDH protein.

Target NameGAPDH

ConjugateUnconjugated

Other NamesG3PD; GAPD; MGC88685

Accession NoSwiss-Prot#:P04406

Uniprot P04406

Gene ID 2597;

Sdspage MW37kd

Concentration1.0mg/ml

FormulationIgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details

Western blotting: 1:5000~1:20000

Western blot analysis of 293T (1), Rat brain (2), NIH 3T3(3), Sheep Muscle(4),Rabbit testis(5), using #37985 diluted at 1:20,000.

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

If you have published an article using product 37985, please notify us so that we can cite your literature.

et al,Aberrant activation of the complement system in renal grafts is mediated by cold storage. In Am J Physiol Renal Physiol on 2021 Jun 1 by Sorena Lo, Li Jiang, et al..PMID:33998295 , (2021),
PMID: 33998295
et al,Hydrogen sulfide-mediated persulfidation regulates homocysteine metabolism and enhances ferroptosis in non-small cell lung cancer. In Mol Cell on 2024 Oct 17 by Hualei Zheng, Huidi Chen, et al..PMID:39321805 , (2024),
PMID: 39321805
et al,The Anti-Senescence Effect and Mechanism of 17β-Estradiol on Pelvic Organ Prolapse Derived Fibroblasts , (2025),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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