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Location： Home > Mouse Monoclonal Antibodies > CD68 Mouse Monoclonal Antibody

CD68 Mouse Monoclonal Antibody#38005

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Product Detail

Product NameCD68 Mouse Monoclonal Antibody

Clone No.6F3

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsIHC,IF

Species ReactivityHu Ms Rt

SpecificityThe CD68 antibody detects endogenous CD68 proteins.

Target NameCD68

ConjugateUnconjugated

Other NamesCD68; CD68 antigen; CD68 molecule; DKFZp686M18236; Gp110; macrophage antigen CD68

Accession NoSwiss-Prot#:P34810

Uniprot P34810

Gene ID 968;

Sdspage MW37kd

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
IHC dilution: 1:200

IF dilution:1:50-200
IHC staining of paraffin-embedded Human tonsil tissue CD68 mouse mAb(6F3) diluted at 1:200.
Immunofluorescence analysis of Human-lung-cancer tissue. 1,CD68 Monoclonal Antibody(6F3)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-spleen tissue. 1,CD68 Monoclonal Antibody(6F3)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,CD68 Monoclonal Antibody(6F3) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,CD68 Monoclonal Antibody(6F3) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.

If you have published an article using product 38005, please notify us so that we can cite your literature.

et al,Preliminary Study on the Antigen-Removal from Extracellular Matrix via Different Decellularization. In Tissue Eng Part C Methods on 2022 Jun by Huan Wu, Guangfu Yin, et al..PMID: 35596569 , (2022),
PMID: 35596569
et al,Celecoxib alleviates denervation-induced muscle atrophy by suppressing inflammation and oxidative stress and improving microcirculation. In Biochem Pharmacol on 2022 Sep by Lilei Zhang, Ming Li, et al..PMID:35882305 , (2022),
PMID: 35882305
et al,SERPINB5 is a novel serum diagnostic biomarker for gastric high-grade intraepithelial neoplasia and plays a role in regulation of macrophage phenotypesInTransl OncolOn2023 NovbyXiuhong Huang 1, Xiaoli Xie et al..PMID: 37573714 , (2023),
PMID: 37573714
et al,Celecoxib ameliorates diabetic sarcopenia by inhibiting inflammation, stress response, mitochondrial dysfunction, and subsequent activation of the protein degradation systems. In Front Pharmacol on 2024 Jan 19 by Chunyan Deng, Chunfeng Lu,et al..PMID:38313305 , (2024),
PMID: 38313305
et al,hBMSC-EVs alleviate weightlessness-induced skeletal muscle atrophy by suppressing oxidative stress and inflammation. , (2025),
PMID: 39901193

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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