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p53 Mouse Monoclonal Antibody#38007

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Product Detail

Product Namep53 Mouse Monoclonal Antibody

Clone No.6C4

Host SpeciesMouse

ClonalityMonoclonal

PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

ApplicationsWB,IHC,IF

Species ReactivityHu

Specificityp53 Mouse monoclonal antibody detects endogenous p53 proteins.

Immunogen DescSynthetic peptide of human p53

Target Namep53

ConjugateUnconjugated

Other NamesTumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13

Accession NoSwiss-Prot#:P04637

Uniprot P04637

Gene ID 7157;

Sdspage MW53kd

Concentration1.0mg/ml

FormulationPBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

StorageStore at -20˚C

Application Details

WB dilution: 1:2000
IHC dilution: 1:200

IF dilution:1:100-200

IHC staining of paraffin-embedded Human colon cancer tissue with p53 mouse mAb(6C4) diluted at 1:200.
IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
Figure legend: Western blot showing p53 (protein code: Tp53) expression in wild-type (WT) and Tp53 shRNA knockdown rat PC-12 cells. Beta-actin (Actb) served as the loading control. Conclusion: Since this antibody detects endogenous p53 protein in WT but not in shRNA knockdown cell lysate, this antibody is highly specific.
Western blot analysis of lysates from 1) 293T, 2) HEK293, 3) A431 cells using p53 Mouse Monoclonal Antibody #38007 (Green) and Actin β Polyclonal Antibody as the loading control (Red).

p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.

If you have published an article using product 38007, please notify us so that we can cite your literature.

et al,Resveratrol suppresses human cervical carcinoma cell proliferation and elevates apoptosis via the mitochondrial and p53 signaling pathways.In Oncol Lett.On 2018 Jun by Li L, Qiu RL et al..PMID:29928358 , (2018),
PMID: 29928358

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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