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ATG7 antibody#38148

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Product Detail

Product NameATG7 antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were purified by affinity purification using immunogen.

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

SpecificityThe antibody detects endogenous level of total ATG7 protein.

Immunogen TypeRecombinant Protein

Immunogen DescRecombinant protein of human ATG7.

Target NameATG7

ConjugateUnconjugated

Other NamesATG7;APG7-LIKE;APG7L;DKFZp434N0735;GSA7;

Accession NoSwiss-Prot#: O95352
NCBI Gene ID: 10533

Uniprot O95352

Gene ID 10533;

Sdspage MW78kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ATG7 at 1:1000 dilution.
Immunofluorescence analysis of NIH/3T3 cells using ATG7 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human breast cancer using ATG7 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ATG7 at dilution of 1:100 (40x lens).

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

If you have published an article using product 38148, please notify us so that we can cite your literature.

et al,Autophagy regulates the degeneration of the auditory cortex through the AMPK-mTOR-ULK1 signaling pathway.In Int J Mol Med. On 2018 Apr by Yuan J, Zhao X et al..PMID: 29344647 , (2018),
PMID: 29344647
et al,Dexmedetomidine Mitigated NLRP3-Mediated Neuroinflammation via the Ubiquitin-Autophagy Pathway to Improve Perioperative Neurocognitive Disorder in Mice. In Front Pharmacol on 2021 May 17 by Lieliang Zhang, Fan Xiao,et al..PMID: 34079457 , (2021),
PMID: 34079457

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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