Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Polyclonal Antibodies > IL10 antibody

IL10 antibody#38392

Datasheet PDF
Availability： The lead time is currently 2 weeks.
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(3) Other
Product Detail

Product NameIL10 antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were purified by affinity purification using immunogen.

ApplicationsWB,IHC

Species ReactivityHuman,Mouse,Rat

SpecificityThe antibody detects endogenous level of total IL10 protein.

Immunogen TypeRecombinant Protein

Immunogen DescRecombinant protein of human IL10.

Target NameIL10

ConjugateUnconjugated

Other NamesIL10; CSIF; GVHDS; IL-10; IL10A; TGIF; Interleukin-10; Cytokine synthesis inhibitory factor;

Accession NoSwiss-Prot#: P22301
NCBI Gene ID: 3586

Uniprot P22301

Gene ID 3586;

Sdspage MW19kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:100
Immunohistochemistry of paraffin-embedded mouse spleen using IL10 at dilution of 1:200 (40x lens).
Western blot analysis of extracts of Jurkat cells, using IL10 at 1:1000 dilution.

IL-10 is an anti-inflammatory cytokine that is produced by T cells, NK cells and macrophages (1,2). IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII (1). Binding of IL-10 leads to the activation of Jak1 and Tyk2, which phosphorylates Stat-3 (1,3). The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFNγ receptor, by upregulating expression of SOCS-1 (1,3). In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28 (4,5). IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy (1). In contrast, increased IL-10 levels contribute to persistent Leishmania major infections (6).

If you have published an article using product 38392, please notify us so that we can cite your literature.

et al,TAM Infiltration Differences in "Tumor-First" and " ZHENG-First" Models and the Underlying Inflammatory Molecular Mechanism in Pancreatic Cancer.In Integr Cancer Ther. On 2018 Sep by Wang FJ, Wang P et al..PMID: 29681184 , (2018),
PMID: 29681184
et al,Inhibition of airway remodeling and inflammation by isoforskolin in PDGF-induced rat ASMCs and OVA-induced rat asthma model.In Biomed Pharmacother on 2017 Nov by Xin Liang, Jingjing Wang,et al..PMID: 28850927 , (2017),
PMID: 28850927
et al,Peptide KLF14-P1 secreted from mesenchymal stem cells protects against bronchopulmonary dysplasia both in vivo and in vitro , (2022),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors