Product Detail
Product NameCUL4A antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were purified by affinity purification using immunogen.
ApplicationsWB,IF
Species ReactivityHuman,Mouse,Rat
SpecificityThe antibody detects endogenous level of total CUL4A protein.
Immunogen TypePeptide
Immunogen DescA synthetic peptide of human CUL4A.
Target NameCUL4A
ConjugateUnconjugated
Accession NoSwiss-Prot#: Q13619
NCBI Gene ID: 8451
Uniprot
Q13619
Gene ID
8451;
Sdspage MW88kd
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB 1:500 - 1:2000
IF 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using CUL4A at 1:500 dilution.
CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins.Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC.
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et al,AMBRA1 drives gastric cancer progression through regulation of tumor plasticity
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