Product Detail
Product NameMLKL antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were purified by affinity purification using immunogen.
ApplicationsWB,IHC,IF
Species ReactivityHuman,Mouse
SpecificityThe antibody detects endogenous level of total MLKL protein.
Immunogen TypeRecombinant Protein
Immunogen DescRecombinant protein of human MLKL.
Target NameMLKL
ConjugateUnconjugated
Other Names9130019I15Rik;
Accession NoSwiss-Prot#: Q8NB16
NCBI Gene ID: 74568
Uniprot
Q8NB16
Gene ID
197259;
Sdspage MW54kd
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using MLKL at 1:1000 dilution.
Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.
If you have published an article using product 38674, please notify us so that we can cite your literature.
et al,ZYZ-803 mitigates endoplasmic reticulum stress-related necroptosis after acute myocardial Infarction through downregulatIng the RIP3-CaMKII signalIng pathway. In Oxid Med Cell Longev on 2019 Jun 2 by Chang L, Wang Z, et al..PMID:31281585
, (2019),
PMID:
31281585
et al,Comprehensive Evaluation of White Matter Damage and Neuron Death and Whole-Transcriptome Analysis of Rats With Chronic Cerebral Hypoperfusion. In Front Cell Neurosci on 2019 Jul 17 by Li W, Wei D, et al..PMID:31379504
, (2019),
PMID:
31379504
et al,Activation of AMPK inhibits Galectin-3-induced pulmonary artery smooth muscle cells proliferation by upregulating hippo signaling effector YAP. In Mol Cell Biochem on 2021 Aug by Qianqian Zhang, Wenge Li, et al..PMID: 33797701
, (2021),
PMID:
33797701
et al,Astragalin Protects against Spinal Cord Ischemia Reperfusion Injury through Attenuating Oxidative Stress-Induced Necroptosis. In Biomed Res Int on 2021 Oct 28 by Feng Sun, Haiwei Zhang, et al..PMID:34746308
, (2021),
PMID:
34746308
The lead time is currently 2 weeks.
