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AKT1S1 antibody#38768

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Product Detail

Product NameAKT1S1 antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were purified by affinity purification using immunogen.

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

SpecificityThe antibody detects endogenous level of total AKT1S1 protein.

Immunogen TypeRecombinant Protein

Immunogen DescRecombinant protein of human AKT1S1.

Target NameAKT1S1

ConjugateUnconjugated

Other NamesLobe; PRAS40;

Accession NoSwiss-Prot#: Q96B36
NCBI Gene ID: 84335

Uniprot Q96B36

Gene ID 84335;

Sdspage MW27kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100

Western blot analysis of extracts of various cell lines, using AKT1S1 at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human mammary cancer using AKT1S1 at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using AKT1S1 at dilution of 1:200 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using AKT1S1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blotting analysis using AKT1S1 antibody #38768.

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003

If you have published an article using product 38768, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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