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SPON1 Antibody#40372

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Product Detail

Product NameSPON1 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntigen affinity purification.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total SPON1 protein.

Immunogen TypePeptide

Immunogen DescSynthetic peptide corresponding to residues near the C terminal of human spondin 1, extracellular matrix protein

Target NameSPON1

ConjugateUnconjugated

Other Namesf-spondin; VSGP/F-spondin

Accession NoSwiss-Prot:Q9HCB6
Gene Accssion:NP_006099

Uniprot Q9HCB6

Gene ID 10418;

Sdspage MW91KD

Concentration1.9mg/ml

FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:200-1:1000
Immunohistochemistry:1:25-1:100
Gel: 6%SDS-PAGE
Lysates (from left to right): Mouse brain and lung tissue
Amount of lysate: 40ug per lane
Primary antibody: 1/400 dilution
Secondary antibody dilution: 1/8000
Exposure time: 2 minutes
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #40372 at dilution 1/30.
F-Spondin, also designated Spondin-1 or vascular smooth muscle growth-promoting factor, is a member of the subgroup of the Thrombospondin type 1 class molecules. F-Spondin is a secreted, extracellular matrix-attached protein which patterns axonal trajectories by promoting adhesion and outgrowth of commissural axons, in addition to inhibiting outgrowth of motor axons.?

If you have published an article using product 40372, please notify us so that we can cite your literature.

et al,FOXO3 regulates Smad3 and Smad7 through SPON1 circular RNA to inhibit idiopathic pulmonary fibrosisInInt J Biol SciOn2023 Jun 12byHailong Li?1,?Jinhe Li et al..PMID:?37416778 , (2023),
PMID: 37416778
et al,Identification of Bone Morphometric Protein-Related Hub Genes and Construction of a Transcriptional Regulatory Network in Patients With Ossification of the Ligamentum FlavumInSpine (Phila Pa 1976)On2023 Sep 15byYifan Tuo?1?2,?Lihong Hu et al..PMID:?37384872 , (2023),
PMID: 37384872
et al,FOXO3 regulates Smad3 and Smad7 through SPON1 circular RNA to inhibit idiopathic pulmonary fibrosis , (2023),
PMID: 37416778
et al,FOXO3 regulates Smad3 and Smad7 through SPON1 circular RNA to inhibit idiopathic pulmonary fibrosis , (2023),
PMID: 37416778

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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