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Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody#40500

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Product Detail

Product NameCleaved-Caspase-3 p17 (D175) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityCleaved-Caspase-3 p17 (D175) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-3 p17 protein resulting from cleavage adjacent to D175.

Immunogen DescSynthesized peptide derived from the Internal region of human Caspase-3 p17.

Target NameCleaved-Caspase-3 p17

ConjugateUnconjugated

Other NamesCASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

Accession NoSwiss-Prot: P42574
NCBI Gene ID: 836

Uniprot P42574

Gene ID 836;

Sdspage MW20kd

Concentration1mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Application Details

Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/100 - 1/300.
Immunofluorescence: 1:100~1:200 
Not yet tested in other applications.

Western Blot analysis of 293 cells using Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody
Immunofluorescence staining of methanol-fixed Human lung cancer cells using Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody #40500.
Immunofluorescence staining of methanol-fixed Mouse liver cells using Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody #40500.
Immunofluorescence staining of methanol-fixed Rat liver cells using Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody #40500.

If you have published an article using product 40500, please notify us so that we can cite your literature.

et al,Baicalin induces cellular senescence in human colon cancer cells via upregulation of DEPP and the activation of Ras/Raf/MEK/ERK signaling.Incell Death Dis. On 2018 Feb 13 by Wang Z, Ma L et al..PMID:29440765 , (2018),
PMID: 29440765
et al,Neuronal SH2B1 attenuates apoptosis in an MPTP mouse model of Parkinson's disease via promoting PLIN4 degradation. In Redox Biol on 2022 Jun by Xiaojuan Han, Yuan Liu, et al..PMID: 35390677 , (2022),
PMID: 35390677
et al,BDNF-TrkB/proBDNF-p75NTR pathway regulation by lipid emulsion rescues bupivacaine-induced central neurotoxicity in ratsInSci RepOn2023;byDanting Jia,#1?Fang Wang et al..PMID:?37884604 , (2023),
PMID: 37884604

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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