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DYKDDDDK Tag(FLAG) Antibody#48107

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Product Detail

Product NameDYKDDDDK Tag(FLAG) Antibody

Clone No.A2-A4

Host SpeciesMouse

ClonalityMonoclonal

PurificationProG affinity purified

ApplicationsWB, IP

Immunogen Descpeptide

ConjugateUnconjugated

Other NamesDDDDK epitope tag antibody
DDDK antibody
ddk antibody
DYKDDDDK antibody
DYKDDDDK epitope tag antibody
DYKDDDDK tag antibody
ECS epitope tag antibody
ECS tag antibody
Enterokinase Cleavage Site epitope tag antibody
Enterokinase Cleavage Site tag antibody
FLAG antibody
FLAG tag antibody

Accession NoSwiss-Prot#:

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:5,000-1:10,000


IP: 1:10-1:50
Western blot analysis on DYKDDDDK Tag recombinant protein (65kDa).
Immunoprecipitation analysis of different lysates using anti-FLAG antibody. Lane 1: Non-transfected 293T Lane 2: Transfected 293T Lane 3: Agarose beads complexes Lane 4: Negative control (Normal mouse IgG)
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.

If you have published an article using product 48107, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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