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GFP (FL) Antibody#48297

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Product Detail

Product NameGFP (FL) Antibody

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsWB;IHC;ICC/IF

Species ReactivityRecombinant protein

Immunogen DescRecombinant Protein of GFP

ConjugateUnconjugated

Other NamesGFP antibody
Green fluorescent protein antibody
yfp antibody

Accession NoSwiss-Prot#:

Uniprot P42212

Calculated MW27 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Application Details
WB 1:1000-1:10000
IHC 1:100-1:500
ICC/IF 1:100-1:500
Western blot analysis of GFP expression in 293 cell lysate transfected with GFP.
Immunofluorescent analysis of 293 cells, using GFP Antibody .
Immunohistochemical analysis of paraffin-embedded GFP transgenic human colon, using GFP Antibody.

Green fluorescent protein(GFP) has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.

If you have published an article using product 48297, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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