Product Detail
Product NamePhospho-I-kappa-B-alpha Antibody
Host SpeciesGoat
ClonalityPolyclonal
PurificationImmunogen affinity purified
ApplicationsWB, IP, IF
Species ReactivityHu, Ms, Rt
Immunogen DescA short amino acid sequence containing Ser 32 phosphorylated IκB-α of human origin.
ConjugateUnconjugated
Other NamesI kappa B alpha antibody I-kappa-B-alpha antibody IkappaBalpha antibody IkB-alpha antibody IKBA antibody IKBA_HUMAN antibody IKBalpha antibody MAD 3 antibody MAD3 antibody Major histocompatibility complex enhancer-binding protein MAD3 antibody NF kappa B inhibitor alpha antibody NF-kappa-B inhibitor alpha antibody NFKBI antibody NFKBIA antibody Nuclear factor of kappa light chain gene enhancer in B cells antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
Accession NoSwiss-Prot#:P25963
Uniprot
P25963
Gene ID
4792;
Calculated MW41kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:100-1:1,000
IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
Western blot analysis of phosphorylated-IκB-α expression in Jurkat (A) and TNFα /ALLN-treated Jurkat (B) whole cell lysates.
On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFκB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to κB sites, suggesting that the IκB-α family binds to the p65 subunit of p50-p65 heterocomplex through Ankyrin repeats. The second member of the IκB family is represented by a protein designated IκB-β. The third group of IκB proteins is represented by IκB-γ, a protein identical in sequence with the C-terminal domain of the p110 precursor of NFκB p50 and expressed predominantly in lymphoid cells. An additional IκB family member has been identified as IκB-ε, a protein which has several phosphorylated forms and is primarily found complexed with RelA and/or c-Rel.
If you have published an article using product 48325, please notify us so that we can cite your literature.
et al,Inhibitory activity of narirutin on RBL-2H3 cells degranulation
, (2021),
PMID:
Yes
