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Location： Home > Polyclonal Antibodies > N-Cadherin Antibody

N-Cadherin Antibody#48495

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Product Detail

Product NameN-Cadherin Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationPeptide affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu, Ms

Immunogen DescSynthetic peptide (KLH-coupled) within human N-cadherin 360-420 aa.

ConjugateUnconjugated

Other NamesCADH2_HUMAN antibody
Cadherin 2 antibody
Cadherin 2 N cadherin neuronal antibody
Cadherin 2 type 1 antibody
Cadherin 2 type 1 N cadherin neuronal antibody
Cadherin 2, type 1, N-cadherin (neuronal) antibody
Cadherin-2 antibody
Cadherin2 antibody
Calcium dependent adhesion protein neuronal antibody
CD325 antibody
CD325 antigen antibody
CDH2 antibody
CDHN antibody
CDw325 antibody
CDw325 antigen antibody
N cadherin 1 antibody
N-cadherin antibody
NCAD antibody
Neural cadherin antibody
OTTHUMP00000066304 antibody
OTTHUMP00000067378 antibody

Accession NoSwiss-Prot#:P19022

Uniprot P19022

Gene ID 1000;

Calculated MW140 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500
IHC: 1:200
ICC: 1:200

FC: 1:50
Western blot analysis of N-cadherin on different tissue lysates using anti- N-cadherin antibody at 1/500 dilution. Positive control:
Lane 1: Mouse heart
Lane2 : Human heart
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-N-Cadherin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-N-Cadherin antibody. Counter stained with hematoxylin.
ICC staining N-Cadherin in 293 cells (red). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining N-Cadherin in mouse embryonic stem cells (green). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining N-Cadherin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
The cadherins are a large family of cell adhesion molecules involved in calcium-dependent recognition and adhesion events. The cadherin subfamily includes N-, P-, B-, and E- cadherins, and other members. Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion. N-Cadherin is commonly found in cancer cells and provides a mechanism for transendothelial migration. When a cancer cell adheres to the endothelial cells of a blood vessel it up-regulates the src kinase pathway, which phosphorylates beta-catenins attached to both N-cadherin (this protein) and E-cadherins.

If you have published an article using product 48495, please notify us so that we can cite your literature.

et al,Neferine, a novel ROCK1-targeting inhibitor, blocks EMT process and induces apoptosis in non-small cell lung cancer. In J Cancer Res Clin Oncol on 2022 Aug 19 by Po Hu, Peng Wan, et al..PMID:35984492 , (2022),
PMID: 35984492
et al,Single-cell sequencing reveals PTX3 involvement in ovarian cancer metastasis. In J Ovarian Res on 2024 Nov 23 by Shuangyan Liu, Tianhao Wu,et al..PMID:39580424 , (2024),
PMID: 39580424

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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