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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Alas1 Rabbit mAb

Alas1 Rabbit mAb#48613

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Product Detail

Product NameAlas1 Rabbit mAb

Clone No.SA70-04

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Names5 aminolevulinate synthase antibody
5 aminolevulinate synthase nonspecific mitochondrial antibody
5 aminolevulinic acid synthase antibody
5-aminolevulinate synthase antibody
5-aminolevulinic acid synthase 1 antibody
Alas 1 antibody
ALAS 3 antibody
ALAS antibody
ALAS H antibody
ALAS HOUSEKEEPING TYPE antibody
ALAS N antibody
ALAS-H antibody
alaS1 antibody
ALAS3 antibody
ALASH antibody
Aminolevulinate delta synthase 1 antibody
Aminolevulinic acid synthase 1 antibody
Delta ALA synthetase antibody
Delta aminolevulinate synthase antibody
Delta-ALA synthase 1 antibody
Delta-aminolevulinate synthase 1 antibody
HEM1_HUMAN antibody
MIG 4 antibody
MIG4 antibody
Migration inducing protein 4 antibody
mitochondrial antibody
nonspecific antibody

Accession NoSwiss-Prot#:P13196

Uniprot P13196

Gene ID 211;

Calculated MW71 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000

ICC: 1:50-1:200

Western blot analysis of Alas1 on different cell lysates using anti-Alas1 antibody at 1/1,000 dilution. Positive control:
Lane 1: JAR
Lane 2: HUVEC
Lane 3: Hela
ICC staining Alas1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Alas1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Alas1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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