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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Filamin A Rabbit mAb

Filamin A Rabbit mAb#48617

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Product Detail

Product NameFilamin A Rabbit mAb

Clone No.SA30-08

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesABP 280 antibody
ABP-280 antibody
Actin-binding protein 280 antibody
Alpha filamin antibody
Alpha-filamin antibody
APBX antibody
CSBS antibody
CVD1 antibody
Endothelial actin binding protein antibody
Endothelial actin-binding protein antibody
Filamin 1 antibody
Filamin A alpha antibody
Filamin A antibody
Filamin-1 antibody
Filamin-A antibody
FLN antibody
FLN-A antibody
FLN1 antibody
FLNA antibody
FLNA_HUMAN antibody
FMD antibody
MNS antibody
NHBP antibody
Non muscle filamin antibody
Non-muscle filamin antibody
OPD antibody
OPD1 antibody
OPD2 antibody
XLVD antibody
XMVD antibody

Accession NoSwiss-Prot#:P21333

Uniprot P21333

Gene ID 2316;

Calculated MW281 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:400
ICC: 1:50-1:200

FC: 1:10-1:100
Western blot analysis of Filamin A on different cell lysates using anti-Filamin A antibody at 1/1,000 dilution. Positive control:
Lane 1: MCF-7
Lane 2: Jurkat
Lane 3: Hela
Lane 4: NIH/3T3
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.
ICC staining Filamin A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Filamin A in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Filamin A in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Filamin A antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in non-muscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of Nebulin are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells.

If you have published an article using product 48617, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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