Product Detail
Product NameBad Rabbit mAb
Clone No.SA32-01
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, ICC/IF, IHC, IP, FC
Species ReactivityHu, Ms, Rt
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesAI325008 antibody
BAD antibody
BAD_HUMAN antibody
BBC 2 antibody
BBC2 antibody
BBC6 antibody
Bcl 2 Antagonist of Cell Death antibody
Bcl 2 Binding Component 6 antibody
BCL X / BCL 2 Binding Protein antibody
BCL X Binding Protein antibody
Bcl XL/Bcl 2 Associated Death Promoter antibody
Bcl-2-binding component 6 antibody
Bcl-2-like protein 8 antibody
Bcl-XL/Bcl-2-associated death promoter antibody
Bcl2 antagonist of cell death antibody
BCL2 antagonist of cell death protein antibody
BCL2 associated agonist of cell death antibody
Bcl2 Associated Death Promoter antibody
BCL2 binding component 6 antibody
BCL2 binding protein antibody
Bcl2 Like 8 Protein antibody
Bcl2-L-8 antibody
BCL2L8 antibody
Proapoptotic BH3 Only Protein antibody
Accession NoSwiss-Prot#:Q92934
Uniprot
Q92934
Gene ID
572;
Calculated MW23 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:10-1:100
Western blot analysis of Bad on MCF-7 cell lysates using anti-Bad antibody at 1/500 dilution.
ICC staining Bad in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Bad in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Bad antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiologic conditions. Bcl-2 and several related proteins function to inhibit apoptosis while other members of the Bcl-2 family, such as Bax and Bak, enhance cell death under various conditions. For instance, Bcl-xL represses cell death, while its shorter form, Bcl-xS, promotes apoptosis. A protein designated Bad exhibits homology to Bcl-2 limited to the BH1 and BH2 domains. Bad functions to dimerize with Bcl-xL and with Bcl-2, but not with Bax, Bcl-xS, Mcl-1, A1 or itself. In mammalian cells, Bad binds with greater affinity to Bcl-xL than to Bcl-2 and reverses the death repressor activity of Bcl-xL but not Bcl-2. Dimerization of Bad with Bcl-xL results in displacement of Bax from Bcl-xL:Bax complexes thereby causing restoration of Bax-mediated apoptosis.
If you have published an article using product 48623, please notify us so that we can cite your literature.
Yes
