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Location： Home > Recombinant Rabbit Monoclonal Antibodies > NeuN Rabbit mAb

NeuN Rabbit mAb#48629

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Product Detail

Product NameNeuN Rabbit mAb

Clone No.SR45-07

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesFLJ56884 antibody
FLJ58356 antibody
Fox-1 homolog C antibody
fox1 homolog C antibody
Fox3 antibody
FOX3NeuN antibody
hexaribonucleotide binding protein 3 antibody
HRNBP3 antibody
NEUN antibody
neuronal nuclei antibody
Rbfox3 antibody
RFOX3_HUMAN antibody
RNA binding protein fox-1 homolog 3 antibody
RNA binding protein, fox 1 homolog (C. elegans) 3 antibody
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Accession NoSwiss-Prot#:A6NFN3

Uniprot A6NFN3

Gene ID 146713;

Calculated MW34 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:200-1:500
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of NeuN on human brain lysates using anti-NeuN antibody at 1/1,000 dilution.( NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NeuN antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-NeuN antibody. Counter stained with hematoxylin.
ICC staining NeuN in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SH-SY-5Y cells with NeuN antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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