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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Rho A+B+C Rabbit mAb

Rho A+B+C Rabbit mAb#48641

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Product Detail

Product NameRho A+B+C Rabbit mAb

Clone No.SR38-00

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAA017882 antibody
AI324259, antibody
Aplysia ras related homolog 9 (RhoC) antibody
Aplysia ras-related homolog 12 antibody
Aplysia RAS-related homolog 6 antibody
Aplysia RAS-related homolog antibody
ARH12 antibody
ARH6 antibody
ARH9 antibody
ARHA antibody
ARHB antibody
ARHC antibody
H12 antibody
H6 antibody
H9 antibody
MGC117867 antibody
MGC1448 antibody
MGC61427 antibody
MST081 antibody
MSTP081 antibody
Oncogene RHO H12 antibody
Oncogene RHO H6 antibody
Oncogene RHO H9 antibody
OTTHUMP00000013675 antibody
OTTHUMP00000013676 antibody
OTTHUMP00000013802 antibody
OTTHUMP00000013805 antibody
OTTHUMP00000013807 antibody
OTTHUMP00000013809 antibody
OTTMUSP00000025086 antibody
OTTMUSP00000025087 antibody
Ras homolog 9 (RhoC) antibody
Ras homolog gene family, member A antibody
Ras homolog gene family, member B antibody
Ras homolog gene family, member C antibody
RAS-related homolog 9 antibody
Rh -related GTP binding protein RhoC antibody
Rho cDNA clone 12 antibody
rho cDNA clone 6 antibody
rho cDNA clone 9 antibody
Rho related GTP binding protein RhoB antibody
Rho related GTP binding protein RhoC antibody
RHO12 antibody
RHOA antibody
RHOB antibody
RHOC antibody
rhoC GTPase antibody
RHOH12 antibody
RHOH6 antibody
RHOH9 antibody
RP11-426L16.4 antibody
small GTP binding protein RhoA antibody
Small GTP binding protein RhoC antibody
Transforming protein RhoA antibody

Accession NoSwiss-Prot#:P08134

Uniprot P08134

Gene ID 389;

Calculated MW24 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:200-1:500
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of Rho A+B+C on different lysates using anti-Rho A+B+C antibody at 1/1,000 dilution. Positive control:
Lane 1: HepG2
Lane 2: Hela
Lane 3: Jurkat
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Rho A+B+C antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Rho A+B+C antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Rho A+B+C antibody. Counter stained with hematoxylin.
ICC staining Rho A+B+C in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Rho A+B+C in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Rho A+B+C in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of HepG2 cells with Rho A+B+C antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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