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Location： Home > Recombinant Rabbit Monoclonal Antibodies > PUMA Rabbit mAb

PUMA Rabbit mAb#48642

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Product Detail

Product NamePUMA Rabbit mAb

Clone No.SR42-09

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesBBC 3 antibody
Bbc3 antibody
BBC3_HUMAN antibody
BCL 2 binding component 3 antibody
Bcl-2-binding component 3 antibody
BCL2 binding component 3 antibody
JFY 1 antibody
JFY-1 antibody
JFY1 antibody
p53 up regulated modulator of apoptosis antibody
p53 up-regulated modulator of apoptosis antibody
p53 Upregulated Modulator of Apoptosis antibody
PUMA alpha antibody
PUMA/JFY1 antibody

Accession NoSwiss-Prot#:Q9BXH1

Uniprot Q96PG8

Gene ID 27113;

Calculated MW18 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of PUMA on different lysates using anti-PUMA antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: K562
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human gastric carcimnoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PUMA antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. Counter stained with hematoxylin.
ICC staining PUMA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PUMA in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with PUMA antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
The expression of PUMA is regulated by the tumor suppressor p53. PUMA is involved in p53-dependent and -independent apoptosis induced by a variety of signals, and is regulated by transcription factors, not by post-translational modifications. After activation, PUMA interacts with antiapoptotic Bcl-2 family members, thus freeing Bax and/or Bak which are then able to signal apoptosis to the mitochondria. Following mitochondrial dysfunction, the caspase cascade is activated ultimately leading to cell death. Several studies have shown that PUMA function is affected or absent in cancer cells. Additionally, many human tumors contain p53 mutations, which results in no induction of PUMA, even after DNA damage induced through irradiation or chemotherapy drugs.Other cancers, which exhibit overexpression of antiapotptic Bcl-2 family proteins, counteract and overpower PUMA-induced apoptosis.

If you have published an article using product 48642, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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