Product Detail
Product NameRab9 Rabbit mAb
Clone No.SR45-05
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, ICC/IF
Species ReactivityHu, Ms, Rt
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other Names2410064E05Rik antibody AI195561 antibody DmRab9 antibody Rab 9 antibody RAB 9A antibody RAB9 member RAS oncogene family antibody RAB9A antibody RAB9A member RAS oncogene family antibody RAB9A_HUMAN antibody RAS ASSOCIATED PROTEIN RAB9 antibody Ras related protein Rab 9A antibody Ras-related protein Rab-9A antibody Sid6061p antibody Sid99 antibody
Accession NoSwiss-Prot#:P51151
Uniprot
P51151
Gene ID
9367;
Calculated MW23 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
Western blot analysis of Rab9 on different lysates using anti-Rab9 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: K562
ICC staining Rab9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Rab9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.
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