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c-Jun Rabbit mAb#48820

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Product Detail

Product Namec-Jun Rabbit mAb

Clone No.SY0298

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB IHC ICC/IF

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesActivator protein 1 antibody
AP 1 antibody
AP1 antibody
cJun antibody
Enhancer Binding Protein AP1 antibody
Jun Activation Domain Binding Protein antibody
JUN antibody
Jun oncogene antibody
JUN protein antibody
Jun proto oncogene antibody
JUN_HUMAN antibody
JUNC antibody
Oncogene JUN antibody
p39 antibody
Proto oncogene c jun antibody
Proto oncogene cJun antibody
Proto-oncogene c-jun antibody
Transcription Factor AP 1 antibody
Transcription factor AP-1 antibody
Transcription Factor AP1 antibody
V jun avian sarcoma virus 17 oncogene homolog antibody
V jun sarcoma virus 17 oncogene homolog (avian) antibody
V jun sarcoma virus 17 oncogene homolog antibody
V-jun avian sarcoma virus 17 oncogene homolog antibody
vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody

Accession NoSwiss-Prot#:P05412

Uniprot P05412

Gene ID 3725;

Calculated MWPredicted band size: 36 kDa

Sdspage MWObserved band size: 43 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: c-Jun Rabbit mAb at 1/1k dilution

Lane 1 : HUVEC whole cell lysates
Lane 2 : 293 whole cell lysates
Lane 3 : HepG2 whole cell lysates
Lane 4 : 3T3 whole cell lysates
Lane 5 : C6 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 43 kDa

Exposure time: 5 seconds
All lanes : c-Jun Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : c-Jun knockdown HEK-293 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast tissue stained for c-Jun using 48820 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat cerebral cortex tissue stained for c-Jun using 48820 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse skeletal muscle tissue stained for c-Jun using 48820 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence c-Jun antibody (48820)

ICC/IF staining of c-Jun in NIH/3T3 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 48820 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is inactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA).

If you have published an article using product 48820, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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