Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Recombinant Rabbit Monoclonal Antibodies > B Raf Rabbit mAb

B Raf Rabbit mAb#48824

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameB Raf Rabbit mAb

Clone No.SU34-04

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesFLJ95109 antibody
94 kDa B raf protein antibody
B raf 1 antibody
B Raf proto oncogene serine threonine protein kinase antibody
B Raf proto oncogene, serine/threonine kinase antibody
B RAF1 antibody
B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody
BRAF 1 antibody
BRAF antibody
BRAF_HUMAN antibody
BRAF1 antibody
cRmil antibody
MGC126806 antibody
MGC138284 antibody
Murine sarcoma viral (v-raf) oncogene homolog B1 antibody
Murine sarcoma viral v raf oncogene homolog B1 antibody
NS7 antibody
Oncogen BRAF antibody
oncogene BRAF1 antibody
p94 antibody
Proto-oncogene B-Raf antibody
Proto-oncogene c-Rmil antibody
RAFB 1 antibody
RAFB1 antibody
RMIL antibody
Serine/threonine-protein kinase B-raf antibody
v raf murine sarcoma viral oncogene homolog B antibody
v raf murine sarcoma viral oncogene homolog B1 antibody
v-Raf murine sarcoma viral oncogene homolog B1 antibody

Accession NoSwiss-Prot#:P15056

Uniprot P15056

Gene ID 673;

Calculated MW85 kDa

Concentration1.15mg/ml

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000
IHC: 1:50-1:200


FC: 1:50-1:100

Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-B Raf antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-B Raf antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-B Raf antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Hela cells with B Raf antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.

If you have published an article using product 48824, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors