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Lactate Dehydrogenase Rabbit mAb#48839

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Product Detail

Product NameLactate Dehydrogenase Rabbit mAb

Clone No.SU39-06

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt, zebrafish

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesCell proliferation-inducing gene 19 protein antibody
GSD11 antibody
L lactate dehydrogenase B chain antibody
L-lactate dehydrogenase A chain antibody
Lactate dehydrogenase A antibody
Lactate dehydrogenase B antibody
Lactate dehydrogenase H chain antibody
Lactate dehydrogenase M antibody
LDH A antibody
LDH B antibody
LDH H antibody
LDH heart subunit antibody
LDH M antibody
LDH muscle subunit antibody
LDH-A antibody
LDH-M antibody
LDH1 antibody
ldha antibody
LDHA_HUMAN antibody
LDHBD antibody
LDHM antibody
MS1111 antibody
PIG19 antibody
Proliferation inducing gene 19 antibody
Renal carcinoma antigen NY REN 46 antibody
Renal carcinoma antigen NY-REN-59 antibody
TRG 5 antibody
TRG5 antibody

Accession NoSwiss-Prot#:P00338

Uniprot P00338

Gene ID 3939;

Calculated MW37 kDa

Concentration1mg/ml

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000-5,000
IHC: 1:200-1:500
ICC: 1:50-1:200

FC: 1:50-1:100

Western blot analysis of Lactate Dehydrogenase on different lysates using anti-Lactate Dehydrogenase antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: A549
Lane 3: MCF-7
Western blot analysis of Lactate Dehydrogenase on hybrid fish (crucian-carp) brain tissue lysate using anti-Lactate Dehydrogenase antibody at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
ICC staining Lactate Dehydrogenase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Lactate Dehydrogenase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Lactate Dehydrogenase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). 

All lanes : Lactate Dehydrogenase Rabbit mAb at 1/1k dilution
Lane 1 : Lactate Dehydrogenase knockout HAP1 cell lysate
Lane 2 : Wild-type HAP1 cell lysate
Lysates/proteins at 20 µg per lane.

The lactate dehydrogenase family (LDH) catalyzes the final step of anaerobic glycolysis, the conversion of L-lactate and NAD to pyruvate and NADH. The LDH family consists of three members, LDH-A, LDH-B and LDH-C, all of which form tetramers consisting four subunits. However, each family member displays a specific tissue distribution pattern with LDH-A and LDH-B predominant in several tissues, specifically LDH-A in muscle and LDH-B in heart, while LDH-C expression is confined to the testis and sperm. LDHs function as powerful markers for germ cell tumors. The genes encoding human LDH-A and LDH-C map to chromosome 11, while the human LDH-B gene maps to chromosome 12. Deficiency in the LDH-A gene is linked to exertional myoglobinuria.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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