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Stathmin 1 Rabbit mAb#48859

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Product Detail

Product NameStathmin 1 Rabbit mAb

Clone No.SS0453

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesC1orf215 antibody
Lag antibody
LAP 18 antibody
LAP18 antibody
Leukemia associated phosphoprotein p18 antibody
Leukemia-associated phosphoprotein p18 antibody
Metablastin antibody
Oncoprotein 18 antibody
OP 18 antibody
Op18 antibody
p18 antibody
p19 antibody
Phosphoprotein 19 antibody
Phosphoprotein p19 antibody
pp17 antibody
pp19 antibody
PR22 antibody
Pr22 protein antibody
Prosolin antibody
Protein Pr22 antibody
SMN antibody
Stathmin antibody
Stathmin1 antibody
STMN 1 antibody
Stmn1 antibody
STMN1_HUMAN antibody

Accession NoSwiss-Prot#:P16949

Uniprot P16949

Gene ID 3925;

Calculated MW18 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Stathmin 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Stathmin 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue using anti-Stathmin 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Stathmin 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Stathmin 1 antibody. Counter stained with hematoxylin.
ICC staining Stathmin 1 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Stathmin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Stathmin 1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Stathmin 1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Op18 (for oncoprotein 18, also designated stathmin, prosolin or metablastin) is a conserved, tubulin-associated, intracellular phosphoprotein . Many different phosphorylated forms of Op18 are observed, and it is expressed as two different isoforms. Op18 is considered a critical regulator of microtubulin dynamics and is downregulated by p53. It serves as a transducing protein, via phophorylation, for a variety of cell signaling pathways and involved in both mitosis and differentiation. Op18 is present in many cancers, including breast carcinomas, and is highly expressed in acute leukemias of different subtypes.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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