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Hexokinase 1 Rabbit mAb#48867

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Product Detail

Product NameHexokinase 1 Rabbit mAb

Clone No.ST47-05

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB IHC ICC/IF FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesBB404130 antibody
Brain form hexokinase antibody
dea antibody
DrHXK1 antibody
EC 2.7.1.1 antibody
Glycolytic enzyme antibody
HEXOKIN antibody
hexokinase I antibody
Hexokinase PI antibody
Hexokinase type I antibody
Hexokinase, tumor isozyme antibody
Hexokinase-1 antibody
Hexokinase-A antibody
HK I antibody
HK1 antibody
HK1 tb antibody
Hk1-s antibody
HK1-ta antibody
HK1-tb antibody
HK1-tc antibody
HKD antibody
HKI antibody
HMSNR antibody
HXK1 antibody
HXK1_HUMAN antibody
im:7148527 antibody
mHk1-s antibody
wu:fc09d08 antibody
wu:fc16e02 antibody
wu:fc21e02 antibody
wu:fq14b11 antibody
zgc:55790 antibody
zgc:77618 antibody

Accession NoSwiss-Prot#:P19367

Uniprot P19367

Gene ID 3098;

Calculated MWPredicted band size: 102 kDa

Sdspage MWObserved band size: 110 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Hexokinase 1 Rabbit mAb at 1/1k dilution

Lane 1 : 293 whole cell lysates
Lane 2 : SH-SY5Y whole cell lysates
Lane 3 : Mouse heart lysates
Lane 4 : Rat liver lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 102 kDa
Observed band size: 110 kDa

Exposure time: 13 seconds
All lanes:Hexokinase 1 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : Hexokinase 1 knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human lung tissue stained for Hexokinase 1 using 48867 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Hexokinase 1 using 48867 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse colon tissue stained for Hexokinase 1 using 48867 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Hexokinase 1 antibody (48867)

ICC/IF staining of Hexokinase 1 in CRC cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 48867 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence Hexokinase 1 antibody (48867)

ICC/IF staining of Hexokinase 1 in MCF-7 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 48867 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Flow Cytometry (Intracellular) Hexokinase 1 antibody (48867)
Flow cytometric analysis of K562 cells with Hexokinase 1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody，red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HXK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines. HXK II is involved in the uptake and utilization of glucose by adipose and skeletal tissues. Of the hexokinases, HXK III has the highest affinity for glucose. Glucokinase is expressed in pancreatic beta cells where it functions as a glucose sensor, determining the set point for insulin secretion.

If you have published an article using product 48867, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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