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Dnmt3a Rabbit mAb#48869

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Product NameDnmt3a Rabbit mAb

Clone No.ST04-78

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF

Species ReactivityHu, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesDNA (cytosine 5) methyltransferase 3 alpha antibody
DNA (cytosine 5) methyltransferase 3A antibody
DNA (cytosine-5)-methyltransferase 3A antibody
DNA cytosine methyltransferase 3A2 antibody
DNA methyltransferase 3 alpha antibody
DNA methyltransferase 3a antibody
DNA methyltransferase HsaIIIA antibody
DNA MTase HsaIIIA antibody
DNM3A_HUMAN antibody
DNMT 3a antibody
DNMT antibody
Dnmt3a antibody
DNMT3A2 antibody
M.HsaIIIA antibody
MCMT antibody
OTTHUMP00000201149 antibody
TBRS antibody

Accession NoSwiss-Prot#:Q9Y6K1

Uniprot Q9Y6K1

Gene ID 1788;

Calculated MW120 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000

ICC: 1:50-1:200

Western blot analysis of Dnmt3a on different lysates using anti-Dnmt3a antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: Human brain
Lane 3: Human heart
ICC staining Dnmt3a in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Dnmt3a in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Dnmt3a in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.

If you have published an article using product 48869, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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