Product Detail
                                
                                                                        Product NameCytokeratin 16 Rabbit mAb
                                        Clone No.SC52-09
                                         Host SpeciesRecombinant Rabbit
                                         Clonality Monoclonal
                                                                                PurificationProA affinity purified
                                        ApplicationsWB, ICC/IF, IHC, FC
                                        Species ReactivityHu
                                                                                                                         Immunogen Descrecombinant protein
                                                                                 ConjugateUnconjugated
                                                                                 Other NamesCK 16 antibody CK-16 antibody CK16 antibody Cytokeratin-16 antibody Cytokeratin16 antibody FNEPPK antibody Focal non epidermolytic palmoplantar keratoderma antibody K 16 antibody K16 antibody K1C16_HUMAN antibody K1CP antibody Keratin 1 type I antibody Keratin 16 antibody Keratin antibody Keratin type I cytoskeletal 16 antibody Keratin-16 antibody Keratin16 antibody KRT 16 antibody Krt16 antibody KRT16A antibody NEPPK antibody PC1 antibody type I cytoskeletal 16 antibody
                                        Accession NoSwiss-Prot#:P08779
                                        Uniprot
                                        P08779
                                        
                                        Gene ID
                                        3868;
                                        
                                        Calculated MW51 kDa
                                                                                                                         Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
                                        StorageStore at -20˚C
                                                                                                                                                         
                                Application Details
                                WB: 1:1,000-1:2,000 
IHC: 1:50-1:200          
ICC: 1:50-1:200
FC: 1:50-1:100
                             
                            
                                                                
                                    
                                    Western blot analysis of Cytokeratin 16 on human skin lysates using anti-Cytokeratin 16 antibody at 1/1,000 dilution.
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.
                                 
                                                                
                                    
                                    ICC staining Cytokeratin 16 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
                                 
                                                                
                                    
                                    ICC staining Cytokeratin 16 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
                                 
                                                                
                                    
                                    ICC staining Cytokeratin 16 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
                                 
                                                                
                                    
                                    Flow cytometric analysis of HepG2 cells with Cytokeratin 16 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
                                 
                                                             
                            
                                Cytokeratins comprise a diverse group of intermediate filament proteins that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, to maintain the overall structural integrity of epithelial cells. Cytokeratins are also useful markers in identifying the origin of metastatic tumors. Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in noninvasive breast carcinomas and normal breast tissue. Mutations in the Cytokeratin 16 gene cause various diseases, including pachyonychia congenita type 1 (PC1), nonepidermolytic palmoplantar keratoderma (NEPPK) and unilateral palmoplantar verrucous nevus (UPVN).
                            
                            
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