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Apg7 Rabbit mAb#48968

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Product Detail

Product NameApg7 Rabbit mAb

Clone No.SC06-30

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Names1810013K23Rik antibody
Apg 7 antibody
APG7 autophagy 7 like antibody
APG7 autophagy 7-like (S. cerevisiae) antibody
APG7 like antibody
APG7, S. cerevisiae, homolog of antibody
APG7-like antibody
APG7L antibody
ATG 7 antibody
ATG12-activating enzyme E1 ATG7 antibody
ATG7 antibody
ATG7 autophagy related 7 homolog (S. cerevisiae) antibody
ATG7 autophagy related 7 homolog antibody
ATG7_HUMAN antibody
Atg7l antibody
Autophagy 7, S. cerevisiae, homolog of antibody
Autophagy related protein 7 antibody
Autophagy-related 7 (yeast) antibody
Autophagy-related protein 7 antibody
DKFZp434N0735 antibody
GSA 7 antibody
GSA7 antibody
hAGP7 antibody
Ubiquitin activating enzyme E1 like protein antibody
Ubiquitin-activating enzyme E1-like protein antibody
Ubiquitin-like modifier-activating enzyme ATG7 antibody

Accession NoSwiss-Prot#:O95352

Uniprot O95352

Gene ID 10533;

Calculated MW78 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of Apg7 on Jurkat cells lysates using anti-Apg7 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
ICC staining Apg7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Apg7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Apg7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
In yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae, and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis specific protein or APS), function as substrates for the autophagy protein APG12. These proteins are covalently bonded together to form APG12/APG5 conjugates, which are required for the progression of autophagy.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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