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Location： Home > Recombinant Rabbit Monoclonal Antibodies > AKAP14 Rabbit mAb

AKAP14 Rabbit mAb#48992

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Product Detail

Product NameAKAP14 Rabbit mAb

Clone No.SC0667

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IP

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesA kinase (PRKA) anchor protein 14 antibody
A kinase anchor protein 14 antibody
A kinase anchor protein 28 kDa antibody
A-kinase anchoring protein 28 antibody
AKAP14 antibody
AKAP28 antibody
PRKA14 antibody
Protein kinase A anchoring protein 14 antibody

Accession NoSwiss-Prot#:Q86UN6

Uniprot Q86UN6

Gene ID 158798;

Calculated MW23 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000

ICC: 1:50-1:200

Western blot analysis of AKAP14 on different lysates using anti-AKAP14 antibody at 1/1,000 dilution. Positive control:
Lane 1: Jurkat
Lane 2: A549
ICC staining AKAP14 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining AKAP14 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining AKAP14 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The protein anchors PKA in ciliary axonemes and, in this way, may play a role in regulating ciliary beat frequency. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Binds to type II regulatory subunits of protein kinase A and anchors/targets them.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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