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Location： Home > Recombinant Rabbit Monoclonal Antibodies > SCF Rabbit mAb

SCF Rabbit mAb#48994

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Product Detail

Product NameSCF Rabbit mAb

Clone No.SC61-06

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesC kit ligand antibody
C-kit ligand antibody
Ckit ligand antibody
DCUA antibody
DFNA69 antibody
DKFZp686F2250 antibody
familial progressive hyperpigmentation 2 antibody
FPH2 antibody
FPHH antibody
KIT ligand antibody
Kitl antibody
KITLG antibody
KL 1 antibody
KL1 antibody
Mast cell growth factor antibody
MGF antibody
MGF stem cell factor antibody
SCF antibody
SCF_HUMAN antibody
SF antibody
SHEP7 antibody
sKITLG antibody
Soluble KIT ligand antibody
Steel factor antibody
steel, mouse, homolog of antibody
Stem cell factor antibody
Stem cell factor precursor antibody

Accession NoSwiss-Prot#:P21583

Uniprot P21583

Gene ID 4254;

Calculated MW37 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:100-1:500
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of SCF on Raji cells lysates using anti-SCF antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SCF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SCF antibody. Counter stained with hematoxylin.
ICC staining SCF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining SCF in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining SCF in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of MCF-7 cells with SCF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Stem cell factor (SCF) is the ligand for the transmembrane tyrosine kinase receptor proto-oncogene c-Kit. SCF, also designated KL, MGF and SLF, is a pleiotropic cytokine that has two alternatively spliced forms, 248 and 220 amino acids in length in human and mouse systems, respectively. Both the larger form and the smaller form are cleaved to produce soluble forms. The smaller splice variant lacks the proteolytic cleavage site, between amino acids 149 and 177 of the larger SCF form. The larger form of SCF is expressed in fibroblasts, brain and thymus, while the smaller form is found in spleen, testis, placenta and cerebellum. The SCF ligand is essential for the development of germ cells, hematopoietic progenitor cells and melanocyte precursors. SCF can stimulate the proliferation of mature mast cells as well as the maturation and proliferation of immature mast cells.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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