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Location： Home > Recombinant Rabbit Monoclonal Antibodies > C/EBP Beta Rabbit mAb

C/EBP Beta Rabbit mAb#49008

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Product NameC/EBP Beta Rabbit mAb

Clone No.SC55-05

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAGP/EBP antibody
C EBP beta antibody
C/EBP beta antibody
C/EBP related protein 2 antibody
CCAAT Enhancer Binding Protein beta antibody
CCAAT/enhancer-binding protein beta antibody
CEBPB antibody
CEBPB_HUMAN antibody
CRP2 antibody
IL 6DBP antibody
IL6DBP antibody
Interleukin 6 dependent binding protein antibody
LAP antibody
Liver activator protein antibody
Liver enriched transcriptional activator antibody
NF IL6 antibody
NFIL6 antibody
Nuclear factor NF IL6 antibody
Nuclear factor NF-IL6 antibody
SF B antibody
SFB antibody
Silencer factor B antibody
TCF-5 antibody
TCF5 antibody
Transcription factor 5 antibody

Accession NoSwiss-Prot#:P17676

Uniprot P17676

Gene ID 1051;

Calculated MW36 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000

ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of C/EBP Beta on different lysates using anti-C/EBP Beta antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: PC-12
ICC staining C/EBP Beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining C/EBP Beta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining C/EBP Beta in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with C/EBP Beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
CCAAT-enhancer binding proteins (C/EBP) are basic region/leucine zipper (bZIP) transcription factors selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP β is a member of the C/EBP transcription factor family. The C/EBP β gene encodes several isoforms containing truncated transcription activation domains due to the alternative translational initiation at multipe AUG start sites. C/EBP β is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein (LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C/EBP β contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C/EBP β gene that has direct implication for acute phase response in liver cells.The C/EBP β form requires phosphorylation for its DNA binding ability, and increase binding of C/EBP β isoforms during acute-phase reaction occurs through its upregulation and structural modification.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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