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Location： Home > Recombinant Rabbit Monoclonal Antibodies > RhoA Rabbit mAb

RhoA Rabbit mAb#49020

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Product Detail

Product NameRhoA Rabbit mAb

Clone No.SN0612

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAplysia ras related homolog 12 antibody
ARH12 antibody
ARHA antibody
H 12 antibody
H12 antibody
Oncogene RHO H12 antibody
Ras homolog family member A antibody
Ras homolog gene family member A antibody
Rho A antibody
Rho cDNA clone 12 antibody
RHO H12 antibody
RHO12 antibody
RHOA antibody
RHOA_HUMAN antibody
RHOH12 antibody
Small GTP binding protein Rho A antibody
Transforming protein Rho A antibody
Transforming protein RhoA antibody

Accession NoSwiss-Prot#:P61586

Uniprot P61586

Gene ID 387;

Calculated MW22 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000-1:2,000

ICC: 1:50-1:200

FC: 1:50-1:100

Western blot analysis of RhoA on rat brain lysates using anti-RhoA antibody at 1/1,000 dilution.
ICC staining RhoA in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with RhoA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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