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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Insulin Receptor Beta Rabbit mAb

Insulin Receptor Beta Rabbit mAb#49107

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Product Detail

Product NameInsulin Receptor Beta Rabbit mAb

Clone No.SN20-13

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic peptide within human Insulin receptor aa 930-970.

ConjugateUnconjugated

Other NamesCD220 antibody
HHF5 antibody
HIR A antibody
INSR antibody
Insulin receptor antibody
Insulin receptor subunit Beta antibody
IR antibody

Accession NoSwiss-Prot#:P06213

Uniprot P06213

Gene ID 3643;

Calculated MW156 kDa

Formulation1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000-1:2,000

ICC: 1:100-1:500

Western blot analysis of Insulin Receptor on different lysates using anti-Insulin Receptor antibody at 1/1,000 dilution. Positive control:
Lane 1: 293
Lane 2: MCF-7
Lane 3: Hela
ICC staining Insulin Receptor in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Insulin Receptor in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Insulin Receptor in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Insulin Receptor in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

The insulin receptor (IR) is a heterodimeric protein complex that has an intracellular β subunit and an extracellular α subunit, which is disulfide- linked to a transmembrane segment. The insulin ligand binds to the IR and initiates molecular signaling pathways that promote glucose uptake in cells and glycogen synthesis. Insulin binding to IR induces phosphorylation of intracellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin. The human insulin receptor gene maps to chromosome 19p13.3-p13.2 and encodes a 1382 amino acid protein that cleaves to form α and β subunits. Type 1 diabetes is an auto-immune condition of the endocrine pancreas that results in destruction of insulin secreting cells and a progressive loss in insulin-sensitive glucose uptake by cells. Type 2 diabetes is a condition where cells become resistant to insulin action.

If you have published an article using product 49107, please notify us so that we can cite your literature.

et al,Neuromechanical Dimorphism of Hypoglycemic Effect of ElectroacupunctureInNeuroendocrinologyOn2023byYun Liu?1,?Tiancheng Xu et al..PMID:?36623492 , (2023),
PMID: 36623492

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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