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Location： Home > Recombinant Rabbit Monoclonal Antibodies > pro Caspase 7 Rabbit mAb

pro Caspase 7 Rabbit mAb#49137

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Product Namepro Caspase 7 Rabbit mAb

Clone No.SD20-57

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB IHC ICC/IF

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Namesapoptosis-related cysteine peptidase antibody
Apoptotic protease Mch-3 antibody
CASP-7 antibody
CASP7 antibody
CASP7_HUMAN antibody
Caspase-7 subunit p11 antibody
CMH-1 antibody
ICE-LAP3 antibody
ICE-like apoptotic protease 3 antibody

Accession NoSwiss-Prot#:P55210

Uniprot P55210

Gene ID 840;

Calculated MWPredicted band size: 34 kDa

Sdspage MWObserved band size: 34 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: pro Caspase 7 Rabbit mAb at 1/1k dilution

Lane 1 : Human lung lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 34 kDa
Observed band size: 34 kDa

Exposure time: 4 seconds
All lanes:pro Caspase 7 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Jurkat cell lysate
Lane 2 : pro Caspase 7 knockdown Jurkat cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon cancer tissue stained for pro Caspase 7 using 49137 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence pro Caspase 7 antibody (49137)

ICC/IF staining of pro Caspase 7 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 49137 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

A unique family of Cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose) polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. Caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.

If you have published an article using product 49137, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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